The are called the teniae coliwhih make up the longitudinal muscle layer of the large intestine
The very inside of the flower, at the center is the ovary. The ovary extends upwards to become the style and then the stigma on top of that. All three of these structures combined are called the pistil. Surrounding the style can be one or more filaments each with an anther on the top which contains the pollen. Around all of those structures are the petals, when combined are the corolla. At the base of the corolla, there are green leaves called calyx.directly under the ovary is the peduncle or stem. Please see the attached diagram for more information.
It would be a young puppy learns to beg for food by watching a older dog
No. Look up what a notochord is, vertebrates cannot have a notochord during embryonic development, as all nutrients flow into the blood stream, or stomach. Notochords are in between the mouth, and stomach. Which would NOT be used during development
Answer:
Use a step wise process
Explanation:
In order to find the presence of the recombinant protein:
- Culture the cells that you believe harbors the plasmid for the recombination protein (+) and also cells that do not (-). This is your control.
- Extract the plasmid from both strains.
- Observe the plasmid map and the area of your insert. Use this to select the restriction enzymes at the beginning and the end of the YFG gene. You can also use a restriction site inside the gene but not in the plasmid for better control.
- Perform the restriction enzyme digest on both (+) and (-) according to your protocol and separate using gel electrophoresis.
- Observe the separate bands that you see. In the (+) there should be a band that is the size of your insert and a larger band that is the size of your plasmid control band and in the (-) there should be a single band that is the size of the plasmid but non the size of the insert.
