Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
it is not possible to access your whole brain.
Answer:
A plant that becomes limp through heat, and loss of water,
Explanation:
Answer: C. Linear chromosomes lacking introns
Explanation: Eukaryotes have linear chromosomes. After cellular replication, introns are spliced in eukaryotes and only keep the exons. This process also includes a 5’ cap and a poly-A tail.
Increasing substrate concentration also increases the rate of reaction to a certain point. Once all of the enzymes have bound, any substrate increase will have no effect on the rate of reaction, as the available enzymes will be saturated and working at their maximum rate.