Gram's staining is a differential staining technique that employs a primary stain like crystal violet and a counter stain like safranin along with the decolourizing agent alcohol and a mordant called the Gram's iodine.
Iodine is a mordant added after the primary stain. It fixes the stain by combining with it to enchance the staining ability. This forms an insoluble crystal violet iodine complex appearing purple under the microscope. These microorganisms are classified as Gram positive.
If addition of iodine is skipped, crystal violet is not fixed on the slide and the insoluble complex is not formed. The cells are decolourized by alcohol and are stained by the counter stain safranin making the Gram positive cells wrongly indentified as Gram negative due to its pink colouration. Thus, the slide will show all the cells as pink coloured Gram negative cells.
Answer:
Cell walls prevent cells from dying in hypertonic conditions.
Explanation:
The cell wall is a rigid structure, essential for the survival of fungi, and knowledge of its composition may be useful for the development of new antifungal drugs. This wall does not alter the characteristics of the fungus, it stimulates and presses the fungus to flourish.
The fungal cell walls are similar in function to the cell walls of many protists, bacteria and plants. They prevent cells from bursting in hypotonic environments, but are unable to prevent cells from dying in hypertonic conditions. These cell walls also provide the cell with a degree of physical environmental protection and differ in the molecular composition of plant cell walls, as the fungal cell walls are chitin, while the plant cell walls are formed by cellulose.
The respiratory and circulatory systems are close together by working and relying with each other. The respiratory system imports oxygen from the air and that oxygen goes through out the body by the circulatory system pumping the oxygen. That creates carbon dioxide, which the respiratory exports from the body.
Coal is a type of organic rock
