Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
The most basic difference is that sea ice forms from salty ocean water, whereas icebergs, glaciers, and lake ice form from fresh water or snow. Sea ice grows, forms, and melts strictly in the ocean. Glaciers are considered land ice, and icebergs are chunks of ice that break off of glaciers and fall into the ocean.
Acids correspond to volatile molecules that release positive hydrogen ions. These ions have a profound effect on the other molecules in foods. Also, they can affect the cells in your body, fortunately, our taste buds help us regulate the number of free hydrogen ions we eat by making us perceive acids as sour tasting.
Answer:
nucleus
Explanation:
eukaryotic has a nucleus, prokaryotic does not
