Gram's staining is a differential staining technique that employs a primary stain like crystal violet and a counter stain like safranin along with the decolourizing agent alcohol and a mordant called the Gram's iodine.
Iodine is a mordant added after the primary stain. It fixes the stain by combining with it to enchance the staining ability. This forms an insoluble crystal violet iodine complex appearing purple under the microscope. These microorganisms are classified as Gram positive.
If addition of iodine is skipped, crystal violet is not fixed on the slide and the insoluble complex is not formed. The cells are decolourized by alcohol and are stained by the counter stain safranin making the Gram positive cells wrongly indentified as Gram negative due to its pink colouration. Thus, the slide will show all the cells as pink coloured Gram negative cells.
The independent variable is the temp because that’s the only thing that changes
dependent is everything else because it doesn’t change
no they aren’t comparing it to any thing, just each other. the control should remain the same throughout the experiment but there isn’t any in this case
light and water because they same through out the experiment
good luck :)
Bacteria are prokaryotic organisms.
Answer: Used for containing genetic and development information
Explanation: DNA, or deoxyribonucleic acid, contains genetic information (genes) used in forming the chromosomes of an organism as well as long term information on how to carry out functions in the organism.
