They are both sites of a new cell form
The layers above would be not as old and the layers below would be the older layers.
An animal cell lacking carbohydrates on the external surface of its plasma membrane would likely be impaired in CELL TO CELL RECOGNITION.
Carbohydrates have diverse functions, one of their functions is that they serve as a recognition signal at the surface of cells.
Carbohydrates located on the surface of cells enable cells to recognize and communicate with one another.
Some of the important questions to be asked should be: Is the procedure of obtaining the results accurate? If not, by how much is its reliability? Are the results useful for further scientific research? What can you recommend to others who might want to make the same scientific conclusions?
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
