Answer:
No, in molecular cloning cutting mistakes can lead to in correct ligation
C) No, because cutting mistakes could lead to incorrect cloning.
Explanation:
in molecular cloning, if you want to insert a specific gene of interest in to specific vector, the selection of enzyme and strategy before that is very important. The restriction enzyme should be selected which restrict at the desired location in the cloning sites. Any mistake in these selection or undesirable mutation mistakes can lead to in correct or unsuccessful cloning results.
Karyotype examination needs to be done at metaphase.
In karyotype test, the examiner is trying to find whether the chromosome is having any abnormality. In metaphase, the chromosome is easier to count and observed.
A chemical (<span>colchicine) will be given to make the cells stuck at metaphase. </span>
