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Talja [164]
3 years ago
8

Students have expressed a protein according to the BASIL protocols. They collected a pre-induction sample, a post-induction samp

le, and a sample following an affinity column purification. The samples were analyzed by SDS-PAGE followed by Coommassie Blue staining. Which lane corresponds to which sample?
Biology
1 answer:
levacccp [35]3 years ago
3 0

Answer:

The first lane with the protein band is the post-induction sample, i.e., the lane with the sample of interest.

Explanation:

Antibody binding is specific and will only bind to whichever protein it has been produced for particularly, which in this case would be the post induction sample.

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It is the bond which is formed by the transfer of electron from one atom to the atom of another element.  

Both bonded atoms have very large electronegativity difference. The atom with large electronegativity value accept the electron from other with smaller value of electronegativity.

For example:

calcium chloride is ionic compound. The electronegativity of chlorine is 3.16 and for calcium is 1. There is large difference is present. That's why electron from calcium is transfer to the chlorine. calcium becomes positive and chlorine becomes negative ion.

There are two valance electrons in calcium so it needed to lose two valance electrons to complete the octet while chlorine needed one electron to complete the octet. Thus two electrons lost by one calcium atom are gained by two atoms of chlorine.

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