Question

PRE-LAB QUESTIONS 1. What is the difference between chemically defined and chemically complex media? Give either a clinical or environmental research example for which each media type would be the most appropriate choice for culturing microorganisms.

Answer 1

Answer:

Explanation a chemical define media is a media that do not contain any animal derived source as a component. It is a media whose chemical component are known. It is said to be the purest form of media for culture.

Complex Chemical media is a media whose component are not known. It includes both animals,yeast, plant source whose component are not known this Mixture makes it complex.

Example of complex media is Human Endothelial-SFM (Life Technologies), Endothelial Basal Media, EndoGRO-LS Complete

Example of chemically defined media enhancing or inhibitory effects of GSLs and sphingolipids on tyrosine kinases associated with growth factor receptors.

Answer 2

Answer: The difference between chemically defined and complex media is that the chemical defined contain an exactly known chemical composition while complex media contain an unknown chemical composition.

Explanation: if we grow microorganisms in laboratories, we must provide them with nutrients and conditions they need in order to get a good growth. There are growth media or culture media for this purpose. The culture medium is a solid, semi-solid or liquid substrate that is rich in nutrients and other necessary elements and supports the growth of microorganisms. We carry out isolation, identification, and maintenance of microorganisms on culture media. There are different types of culture media depending on the composition and use. Chemically defined media and complex media are two main types of these. Chemically defined media contain pure biochemical; hence, the chemically defined media contain exactly known chemical composition. However, complex media contain complex materials such as blood, milk, beef extract, etc., so the chemical composition of the complex media is unknown.

Chemically defined media are useful when studying the minimal requirements of microorganisms and for a variety of physiological studies. Furthermore, these media are useful in in vitro cell cultures of human and animal cells. Glucose salt broth is one example of such media. Peptone broth is another example.

Chemically complex media help to grow microorganisms that have complex nutritional requirements.

Furthermore, complex media are usually used for the cultivation of bacterial pathogens and other fastidious bacteria. Nutrient broth/agar, tryptic soy broth/agar, and blood agar are a few examples of complex media.

Good luck