We need to reverse transcribe the mRNA to cDNA before PCR and NGS sequencing because the RNA can not be used by the Taq polymerase.
Enzymatic conversion of mRNA into double-stranded insert DNA can be finished via a number of special techniques. they all involve the movement of reverse transcriptase and oligonucleotide-primed synthesis of cDNA. After that, the approaches in commonplace use diverge drastically.
Messenger RNA (mRNA) vaccines educate our cells on the way to make a protein so one can trigger an immune response internal our bodies. Researchers had been reading and working with mRNA vaccines for decades.
cDNA (short for copy DNA; also known as complementary DNA) is artificial DNA that has been transcribed from a specific mRNA via a response using the enzyme opposite transcriptase. while DNA is composed of both coding and non-coding sequences, cDNA consists of the most effective coding sequences.
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Answer:
Lifestyle segmentation
Explanation:
In the context of psychographic segmentation, lifestyle segmentation is the one that includes what someone values and how people spend their time and money. It analyses three aspects of someone's lifestyle: activities, interests, and opinions, also known as the 'AIO variables'.
The lifestyle segmentation, therefore, includes the activities a person enjoys and will spend money on, how much they enjoy those activities and the method they use when they spend money on those activities.
The main focus of the activities of my staff will be on how teens can have acceptable behaviors which can be used for nation building and how they can contribute positively to the nation.
Youths will be engaged in several programs within the community. These programs will recognize their strengths, and help shape them for the future.
Youths will be taught about the importance of good behaviors and the negative effects of bad behavior in the society to enable them understand that only good behavior are essential for their development as an individual and as a nation as a whole.
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If I was the construction worker I would get tools such as “ pickaxe, chisels, granite hammers, dolerite, and other stone tools to help me brake and shape things. Then I will bring a will barrow to help move have objects around the place.
Answer:
Phoenicia from Ancient Greek:
was an ancient Semitic-speaking thalassocratic civilization that originated in the Levant region of the eastern Mediterranean, primarily modern Lebanon.[5][6] It was concentrated along the coast of Lebanon and included some coastal areas of modern Syria and Galilee (northern Palestine), reaching as far north as Arwad and as far south as Acre and possibly Gaza.[7][8][9] At its height between 1100 and 200 BC, Phoenician civilization spread across the Mediterranean, from the Levant to the Iberian Peninsula.
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