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-Dominant- [34]
2 years ago
15

Why do we need to reverse transcribe the mrna to cdna before pcr and ngs sequencing?

Social Studies
1 answer:
Anestetic [448]2 years ago
3 0

We need to reverse transcribe the mRNA to cDNA before PCR and NGS sequencing because the RNA can not be used by the Taq polymerase.

Enzymatic conversion of mRNA into double-stranded insert DNA can be finished via a number of special techniques. they all involve the movement of reverse transcriptase and oligonucleotide-primed synthesis of cDNA. After that, the approaches in commonplace use diverge drastically.

Messenger RNA (mRNA) vaccines educate our cells on the way to make a protein so one can trigger an immune response internal our bodies. Researchers had been reading and working with mRNA vaccines for decades.

cDNA (short for copy DNA; also known as complementary DNA) is artificial DNA that has been transcribed from a specific mRNA via a response using the enzyme opposite transcriptase. while DNA is composed of both coding and non-coding sequences, cDNA consists of the most effective coding sequences.

Learn more about mRNA here brainly.com/question/24885193

#SPJ4

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What are the advantages of analyzing and experimenting with a model as opposed to a real object or situation
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The advantages of analyzing and experimenting with a model is that the outcome will be significantly less "real" than a real object as well as the fact that you will know the difference between the two (model & real) and what will happen.

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A culture in which public space and private space are similar in size and individuals guard their public space carefully, becaus
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diffuse culture.

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Diffuse culture is one in which people prefer and understandindirect communication. This is in a bid to limit exposure of their public space.

The belief is that those having access to their public space can also access their private space.

For example a parent in the upper class conveying to their child that they cannot marry from a lower class.

This behaviour limits public space in order to protect private space. So they are similar in size.

Countries that have diffuse culture include: China, Russia, India, Spain, and Argentina.

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TRUE!

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D) Periodically measure how the treatment is being implemented.

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- Hope this helps. Have a great day!

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