Why do we need to reverse transcribe the mrna to cdna before pcr and ngs sequencing?

1 answer:

3 0

We need to reverse transcribe the mRNA to cDNA before PCR and NGS sequencing because the RNA can not be used by the Taq polymerase.

Enzymatic conversion of mRNA into double-stranded insert DNA can be finished via a number of special techniques. they all involve the movement of reverse transcriptase and oligonucleotide-primed synthesis of cDNA. After that, the approaches in commonplace use diverge drastically.

Messenger RNA (mRNA) vaccines educate our cells on the way to make a protein so one can trigger an immune response internal our bodies. Researchers had been reading and working with mRNA vaccines for decades.

cDNA (short for copy DNA; also known as complementary DNA) is artificial DNA that has been transcribed from a specific mRNA via a response using the enzyme opposite transcriptase. while DNA is composed of both coding and non-coding sequences, cDNA consists of the most effective coding sequences.

Learn more about mRNA here brainly.com/question/24885193

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