We need to reverse transcribe the mRNA to cDNA before PCR and NGS sequencing because the RNA can not be used by the Taq polymerase.
Enzymatic conversion of mRNA into double-stranded insert DNA can be finished via a number of special techniques. they all involve the movement of reverse transcriptase and oligonucleotide-primed synthesis of cDNA. After that, the approaches in commonplace use diverge drastically.
Messenger RNA (mRNA) vaccines educate our cells on the way to make a protein so one can trigger an immune response internal our bodies. Researchers had been reading and working with mRNA vaccines for decades.
cDNA (short for copy DNA; also known as complementary DNA) is artificial DNA that has been transcribed from a specific mRNA via a response using the enzyme opposite transcriptase. while DNA is composed of both coding and non-coding sequences, cDNA consists of the most effective coding sequences.
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