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gulaghasi [49]
3 years ago
8

Air rushes into the lungs of humans during inhalation because _____.

Biology
1 answer:
Delvig [45]3 years ago
3 0
Because the rib muscles and diaphragm contract,increasing the lung volume
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In colorectal cancer, some tumor suppressor genes are inactive. This is an important factor resulting in uncontrolled cell divis
tresset_1 [31]

Answer/Explanation:

(1) a mutation in the coding region, resulting in an inactive protein

To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.

To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.

(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.

To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.

To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR

3 0
3 years ago
Fill in the missing information for each Punnett square below:
kow [346]
I filled in each labeled square for you and wrote each percentage to the right of each square with the genotype of the last two.

4 0
3 years ago
By signing the Kyoto Protocol, a country commits to decrease its greenhouse gas emissions. Which ecological issue does this prot
Otrada [13]

Answer:

it addresses Loss of Biodiversity

Explanation:

if you read the question you find a key word which is decrease

hope this helped

4 0
3 years ago
What is an agro-forestry?
Sliva [168]

Answer:

Agroforestry is a collective name for land-use systems and technologies where woody perennials (trees, shrubs, palms, bamboos, etc.) are deliberately used on the same land-management units as agricultural crops and/or animals, in some form of spatial arrangement or temporal sequence.

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2 years ago
Earth’s outermost layer is separated into a dozen or more large and small slabs, called _______.
Nadya [2.5K]
A tectonic plates

Because the outermost part of earth is know as the lithosphere. The lithosphere is divided into a number of tectonic plates. These plates move and interact with one another, driven by conventional forces within the earth
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