Answer:
In order to study an enzyme, or any trait of an extremophile microorganisms, you must try to replicate their natural ennvironment conditions. It means, you need to approach the 100 °C temperature, and measure this acidic pH to be sure which you need to use for your experiment. Besides, if it is suspected that the protective coating production is catalized by this enzyme, you really need to stress the cells soy they produce it.
Explanation:
I t is the type of promoter
The two main points from Darwin's theory of evolution is that 1) most, if not all living organisms evolved from one or just a few common ancestors and that subsequent specialization of further generations then led to new species. Furthermore, he said that 2) the process how this was done was by natural selection where the organisms which were most adapted to their environment were successfully 'remembered in the gene pool'.