Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
<span>Warbler Finches are most like the ancestral finch</span>
The correct answer is:
a. Jupiter
Explanation:
In 1610, Galileo Galilei turned his rudimentary telescope on Jupiter and understood that it had 4 large moons orbiting it: Io, Europa, Ganymede, and Callisto. This was an amazing discovery because it explained that Earth was not the center of the Universe as champions of the geocentric view assumed.
The correct answer is: Galaxies in the universe are moving away from the earth.
Well it really depends on what kind of change there is. For this I'll use a drought as an example. If a drought occurs in an area the plant life and vegetation will start to die off in that area. From there, primary consumers would run out of food. then secondary consumers, and so forth and so on. it'd be like putting a cow in a field of dirt with no food and a large bucket of water. Eventually the cow will die of starvation. Now apply this to all of the animals in that ecosystem.
