Virus can reproduce only within a host cell, this cycle of infection begins with the <em>attachment</em>, where the virus attaches to a specific receptor site on the host cell, after this comes the<em> entry,</em> in the case of enveloped virus, the envelope can fuse directly with the cell membrane to enter the cell, they can also enter through endocytosis. After entering the cell the virus initiates a <em>replication and assembly </em>mechanism depending on its genome, finally, the last stage of viral replication is the <em>release or egress </em>of the new virions produced in the host organism, some viruses can be released when the host cell dies, but some can leave infected cells by budding through the membrane without directly killing the cell.
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If the alcohol concentration in PEA agar was increased, it would inhibit both Gram - and Gram + bacteria by breaking down membrane absorbency barrier, tolerating influx of substances that are generally blocked and leakage of cellular potassium. High alcohol concentrations would lead to disruption or halting of DNA synthesis in both Gram + and Gram - bacteria
