Answer:
Stablising
Explanation:
Bcoz they acquiring mean character
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
2.
Explanation:
The movement of sediments from water (rivers) and wind (air) is basically what erosion is.
Answer: lower atmospheric pressure
Explanation:
During the inspiration process the diaphragm, and external intercostal muscles. The contraction of the diaphragm causes the increase in the size of the thoracic cavity this causes the contraction of the external intercostal muscles. As a result of this the ribs and the sternum get elevated.
This causes the lungs to expand, and increasing the volume of the air passage. In this response the air pressure inside the lungs reduces below the air outside the body. Thus this facilitates the movement of the gases from a region of high pressure to low pressure, this way the air rushes into the lungs.
