Answer:
In the decolorization step alcohol is used which destain the gram-negative bacteria but not gram-positive because alcohol degrades the LPS cell wall of gram-negative bacteria which allow the crystal violet to moves out of the cell.
So if we use too little alcohol then the alcohol will not able to decolorize all the gram-negative bacteria and most of the cell in the culture will appear violet or purple because crystal violet retained in the cell wall of bacteria.
So the sample of gram-negative bacteria will appear as gram-positive bacteria under the microscope.
The correct form to write a binomial nomenclature is Genus species
It could cause an accident and hurt yourself or others
The fertilized ovule becomes the seed, and the ovary becomes the fruit.
