Answer:
1) Sterilize the loop.
2)Flame lip of TSS culture with <em>S. aureus</em>.
3) Remove some bacteria from surface of TSS culture with <em>S. aureus.</em> Remove loop.
4)Flame lip of TSS culture with <em>S. aureus</em>.
5)Flame lip of broth culture tube.
6) Dip loop into sterile TSB and swirl. Remove loop.
7)Flame lip of broth culture tube.
8) Sterilize the loop.
Explanation:
The aseptic technique is a s<u>eries of steps to prevent contamination during manipulations of cultures and sterile culture media</u>. A mastery of aseptic technique is required for maintaining pure cultures, as airborne contaminants are virtually everywhere. Picking an isolated colony and restreaking it is the main method for obtaining pure cultures from liquid samples containing several different organisms and is a common procedure in the microbiology laboratory. Other techniques for obtaining pure cultures have been developed that are especially suited for particular groups.
