Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
In general, there are three types of Cell division :
- Binary fission, a division process on which a cell is completely cloned, forming 2 exact same cells
- Mitosis , a division of a somatic cell of Eukaryotic organisms
- Meioses, a cell division that produces gametes
hope this helps
The priority after administering is to assess fetal heart
rate. After administering an opioid to a laboring mother, the main concern is
to evaluate the effect on the fetus. Opioid administration can cross the
placental obstruction with signs as well as measuring heart rate and
variability. Subsequently after birth, there may be a reduction in attentiveness.
Maternal factors of a reduced blood pressure, constipation and dry month are of
a lesser importance.
Answer:
Weathering describes the breaking down or dissolving of rocks and minerals on the surface of the Earth. Water, ice, acids, salts, plants, animals, and changes in temperature are all agents of weathering.
Once a rock has been broken down, a process called erosion transports the bits of rock and mineral away. No rock on Earth is hard enough to resist the forces of weathering and erosion. Together, these processes carved landmarks such as the Grand Canyon, in the U.S. state of Arizona. This massive canyon is 446 kilometers (277 miles) long, as much as 29 kilometers (18 miles) wide, and 1,600 meters (1 mile) deep.
Weathering and erosion constantly change the rocky landscape of Earth.
Explanation:
HOPE IT HELPS. PLS MARK AS BRAINLIEST
Answer:
a. People with celiac disease should include foods with dietary fiber such as beans, fruits, vegetables, brown rice, and quinoa.
e. Home-cooked meals are a good way to increase variety and reduce the cost of a gluten-free diet.
Explanation:
Celiac disease is an autoimmune disease, as stated above. This disease causes the defense cells of an organism to attack the stomach cells causing an inflammation that is extended and stimulated by the glutem. As a result, the individual has diarrhea, constipation, weight loss, anemia, a feeling of fullness, colic, abdominal discomfort, severe pain and may even develop ulcers and cancers.
Glutem is a protein found in wheat, barley and rye and for that reason, individuals with this disease should avoid these foods or foods derived from them. This type of diet should accompany the sick person for life and they should be encouraged to eat homemade foods that know the origin and composition. In addition, it is necessary that these people feed on dietary fibers, such as beans, fruits, vegetables, brown rice and quinoa.
