Question

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens. However, upon testing it out against a known Neisseria gonorrhoeae culture, you find that it does not work. Which of the following is a possible explanation for this negative result? A.You forgot to label the probe sequence with a reporter molecule.
B. You forget to digest the probe with restriction endonucleases

C. You forget to add the mRNA to the DNA probe sample.

D. You forgot to incubate the test at the 75C temperature required for hybridization.

Answer 1

Answer:

The correct answer is i forgot to label the probe with a reporter molecule.

Explanation:

Reporter molecule is a specific type of molecule that reports the success of cloning.

      In this experiment to test the presence of N gonorrhoeae by using the DNA probe that was developed by me should be at first labelled probe sequence with a reporter gene such as lucciferase protein.

       When this reporter molecule labelled DNA probe will bind to the target DNA sequence of N gonorrhoeae at that time by using the substrate specific for that reporter molecule will help me to notice the process of hybridization.