Restriction enzymes refer to the class of enzymes which are involved in cleaving the DNA molecule at or near a particular site which is termed as a restriction site.
Gene of interest and bacterial DNA are treated with the same restriction enzyme (mostly restriction endonuclease) which help in producing sticky ends in the gene of interest as well as bacterial DNA.
The digestion with restriction enzyme helps in removing the gene of interest from other human DNA. In addition, it opens the circular bacterial DNA.
Lastly, the sticky ends help in inserting the gene of interest into the bacterial DNA.
All the important functions of our body would simply be too slow for us to survive such as digestion. if we didn't digest quick enough our stomachs would be bloated all the time
Sound waves need a medium to travel through. That's why you cant hear sound in space. But you can see light in space and the sun heats up our planet, so yes, light doesnt need a medium to travel through.