Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Don not know sorryyyyyyyyyuyyyyyyy
Answer:
It depends on the structure of labeled A.
Explanation:
If the diagram shows the structure of flower, so we can say that it has the ability to attract insects and other animals. If the diagram shows the structure of an insect such as bees so we can say that it can moves the pollen to the ovaries. If the diagram indicates the structure of pistil which is a female part of the flower so we choose that it catches the pollen and if the diagram represents the structure of anther which is a female part so we choose that it produces pollen.
I believe that glyceraldehyde-3-phosphate (G3P) is a product of the Calvin cycle that is used to form glucose phosphate amino acids or fatty acids.
The Calvin cycle is divided into ; carbon fixation, reduction and regeneration of the starting molecule. Energy from ATP and from the reduced coenzyme NADPH is used to remove a phosphate group from 3PGA and reduce the resulting diphosphoglycerate (DPGA) to produce the 3-carbon sugar glyceraladehyde-3-phosphate (G3P).
