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Lorico [155]
3 years ago
10

A solvent passes through a chromatography column in 3.0 minutes, but the solute requires 9.0 minutes.

Physics
1 answer:
Sergio [31]3 years ago
4 0

Answer:

(a). 2

(b). 1/3

(c). 11.11

Explanation:

(a).  k= (t₍s₎-t₍o₎)/t₍o₎...............(1)

where k= retention factor,

t₍o₎=solvent time, t₍s₎= solute time.

Given t₍s₎=9.0 Minutes, t₍o₎=3.0 minutes.

∴ k= (9-3)/3

  k= 2.

(b). the fraction of time the solute spend in the mobile phase in the column is the ratio of the solvent time to the solute time. = t₍o₎/t₍s₎..........(2)

= 3/9

=1/3.

(c). K=k(Vm/Vs)................(3)

where K= partition coefficient, k= retention factor, Vm=volume of mobile phase, Vs= volume of stationary phase.

∴K = k(Vm/Vs)

k=2, and Vs=0.18Vm.

∴K = 2(Vm/0.18vm)

⇒K = 2/0.18

∴K=11.11

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