Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
When looking at a Neuromuscular Junction (NMJ), we know that neurotransmitters (NT) are released from the presynaptic cell and they then bind to the receptors that are located on the postsynaptic cell - this causes the effect of the NT being released.
So we are told that NT are still being released, however they are not having an effect. This would mean that they are probably being blocked by something - in this case, it seems that the neurotoxin is the culprit in the blocking of these receptors.
Therefore, if the NT cannot bind to the receptors on the postsynaptic cell, they are not going to have any effect, no matter how much NT is being released.
So the answer in this case is: The neurotoxin is most likely C) Blocking the receptors on the postsynaptic cell.
Answer:
Eosinophils
Explanation:
The exact role of eosinophils in your body isn't clear, but they're usually found attacking allergic diseases and certain infections
Answer:
- Option D is the correct answer.
Explanation:
It is given that a 2 kg ball is traveling at a speed of 4 m/s, so we have to find its kinetic energy.
KE = 1/2 mv²
KE = 1/2 × 2×(4)²
KE = 1/2 × 2×16
KE = 16 J
<u>Some information about Kinetic Energy</u>.
Kinetic Energy is the energy an object has because of its motion. It is directly proportional to the velocity of the particle and its mass. Kinetic energy is given by,
- KE = ½mv²
- It occurs due to the motion of the particle.
- If a particle at rest position, then the kinetic energy will be zero because that particle won't be accelerating.
the exploitation of biological processes for industrial and other purposes, especially the genetic manipulation of microorganisms for the production of antibiotics, hormones,...
