During protein electrophoresis set up at a buffer pH of 8.6, proteins are __ charged and migrate toward the

Question

rusak2 [61]

4 years ago

6

During protein electrophoresis set up at a buffer pH of 8.6, proteins are __ charged and migrate toward the

___:

Chemistry

1 answer:

iren2701 [21] · Answer 1 · 2022-01-21T13:51:11+03:00

Answer: negatively charged; positively charged electrode.

Explanation:

Electrophoresis can de defined as the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. The electrophoresis of negatively charged particles is called anaphoresis while the electrophoresis of positively charged particles is called cataphoresis.

During proten electrophoresis, due to electric field, proteins migrate through pores in a polyacrylamide gel matrix. It must be noted that pore size decreases with increasing acrylamide concentration.

Proteins set up at a buffer pH of 8.6, the proteins will become negatively charged (that is, anions) and it will move to the positively charged electrode.

During protein electrophoresis set up at a buffer pH of 8.6, proteins are ____________ charged and migrate toward the __________

During protein electrophoresis set up at a buffer pH of 8.6, proteins are __ charged and migrate toward the