Answer: B. Melting temperatures of primer should be between 55-80 degree Celsius.
Explanation:
Bacause the melting temperature controls the binding of the primers to your template DNA. At melting temperature 50% of the primer molecules are bound to their corresponding target sequence. If the difference in melting temperature between the two primers is too high, it might be difficult to find experimental conditions where both primers can bind to their target.
Answer: close ended
Explanation:
In a job oriented interview both open and closed ended questions are asked to the job seeker. The open ended interview questions are generally very long answer type questions these questions allows the person to express. These questions are asked to learn about the personality and interests of the job seeker. The closed ended questions are the short questions and they are easy to answer and these can be answered in yes and no. These questions are asked and answered quickly. The chances of mistakes in these questions are more. The chances of misinterpretation of the question is always more in these questions. But these questions allow to get more information about the candidate as compared to the open ended questions as during the open ended question session the job seeker can manipulate the facts but in the case of close ended questions the asking tendency is rapid so no manipulation can occur.
The greatest difference in high and low tides occur during spring tides when the sun and moon are aligned
I think its Multicellular Organisms,., i could be wrong
Answer:
Mass does not affect gravity and distance has an indirect relationship.
Explanation:
The equation for gravity is, g = 
where, G = gravitational constant
M = mass of the planet
R = distance between the bodies (object and center of planet)
so, gravity depends on the mass of planet, not on mass of object
and is inversely proportional to sqaure of distance.
