The answer is jellyfish B
Chinese hamster ovary cell production of recombinant tissue‐type plasminogen activator (t‐PA) was increased by amplification of cotransfected dihydrofolate reeducates cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 μM MTX and yielded 26,000 U/106 cells/day t‐PA (43 μg/106 cells/day). Above 25 μM MTX, cell specific t‐PA production rates became increasingly variable and the cDNA copynumbers decreased. No apparent correlation between the cell specific t‐PA production rate and the growth rate was observed upon sub cloning of the amplified cells. When MTX selection was removed, the t‐PA production rate decreased up to tenfold within 40 days; this was accompanied by an up to 60% drop in cDNA copynumber. Subclones isolated after 108 days of culture in the absence of MTX were, on average, sixfold more stable than their parental cells. In culture without MTX, the maximum stable t‐PA production rate obtained (over 250 days) was 7000 ± 750 U/106cells/day (∼12 μg/106 cells/day), approximately threefold lower than the maximum unstable levels of production reached under selective pressure. Taken together, these results define a wide range of the highest t‐PA expression rates obtained under MTX selection, for which stable expression without selection has not been reported
Pseudomonas aeruginosa is of concern in burn patients beacuse it can cause infections like AIDS,diabetes mellitus , etc.
Pseudomonas aeruginosa is a common bacteria that can be found in soil, water, and other environmental niches. Burn wounds are intricate microenvironments where bacterial pathogens like P. aeruginosa can cause infections. Typical virulence factors like pyocyanin and pyoverdine expressed at higher levels than they would have under conventional laboratory conditions.
Elastase is secreted as well, is primarily responsible for the host tissues' degradation. P. aeruginosa infections cause severe consequences mostly in organisms with weakened natural defences. Examples of these circumstances include burn wound patients.
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Add all your numbers up and subtract form the smallest number and its PH2
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