Answer:
The correct answer is -
II: lyse cells of interest, precipitate DNA
V. Digest (cut) genomic DNA with restriction enzyme
I. Digest (cut) phage (chromosome) with restriction enzyme so can ligate in the gene in next step!!
IV. Perform ligation (glue plasmid in!) reaction with genome fragments and phage.
III Introduce constructs via transformation into competent E. coli cells.
Explanation:
The total collection of the genomic DNA of an organism is known as the genomic library of the particular organism. In this library, the genomic DNA is inserted and stored in identical vectors.
The genomic library construction involved some major steps that are:
- Extract and purify DNA - lysing and separating the desired DNA of interest.
- Digest the DNA with a restriction enzyme - breaking or digesting DNA into fragments with restriction enzymes
- cut phage (chromosome) with restriction enzyme
- Insert the fragments of DNA into vectors
- introduce these recombinant a host bacterium by transformation, creating a DNA library.
Answer:
Steam.
The burning coal warms water in a boiler, resulting in the production of steam. Steam from the boiler rotates the turbine engine's blades, converting thermal energy from the burning coal into mechanical energy that powers the turbine engine.
Hope this helps
Have a nice day sir or ma'am! :D
Please give brainliest
1- Emigration and immigration rate
2- Death rate and birth rate
These factors determine and affect the number of organisms of a species in a particular community. Hope this helps!
Sam drives an excavator at an open surface strip coal mine. He has the most tendency to collect the lignite coal which is found in the outermost surface of the earth.
<h3>
What is Lignite?</h3>
The brown coal known as lignite, which is found in the topmost layer of the earth's crust, is frequently referred to as such and is essential for commercial use due to the fact that these coals are the only ones used for the majority of applications.
Electricity produced from lignite is plentiful, affordable, dependable, and environmentally friendly. Lignite is a flammable mineral that ranges in color from dark brown to black and was created over millions of years by the partial decomposition of plant matter under conditions of elevated pressure and temperature in an atmosphere devoid of air.
To learn more about lignite visit:
brainly.com/question/16421194
#SPJ4
Answer: Nucleus membrane isn't part of the ER or Golgi Apparatus, BUT all of them are part of the endomembrane system.
Endoplasmic reticulum and Golgi apparatus are two membrane-bound organelles found in eukaryotes. Both these organelles are closely associated and functionally related. Both endoplasmic reticulum (ER) and Golgi apparatus are two components of the endomembrane system of a cell. Lysosomes and vesicals are the other components of the endomembrane system.
