the correct answer is A- Reproductive isolation occurred through behavioral isolation
A process of formation of a new species from an existing species is called speciation. New species may formed due to geographical or reproductive barriers which isolates the members of a population of a species to form a new species.
In the given question, speciation in In the leopard frogs occurred due to a particular or specific call given by males to the females. The courtship call lead to reproductive isolation due to change in behavior. Such type of behavioral change will help in recognizing mates of the same species and this will act as a reproductive barrier among the other related species.
Limiting factors are resources or other factors in the environment that can lower the population growth rate. Limiting factors include a low food supply and lack of space. Limiting factors can lower birth rates, increase death rates, or lead to emigration.
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