Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3, ~6 ~10.
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The higher the GC content of a DNA, the higher the strength of the interchain interactions, and the higher the melting temperature.
DNA is Deoxyribonucleic Acid. It is the genetic material in maximum of the organisms. The DNA is a polymer made from nucleotides (sugar, nitrogenous bases and phosphate group). It is composed of two strands of phosphate and sugar backbone in an antiparallel direction. The nitrogenous bases present in DNA are Adenine (A), Thymine (T), Guanine (G) and Cytosine (C).
GC content refers to the total amount of Guanine and Cytosine present in the DNA. Since there exists a triple bond between G and C, more percentage of them provides strength to DNA. That is why high GC content denotes high melting temperature of DNA.
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