Answer:
Cut open the plasmid and "paste" in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA).
Insert the plasmid into bacteria. Use antibiotic selection to identify the bacteria that took up the plasmid.
Grow up lots of plasmid-carrying bacteria and use them as "factories" to make the protein. Harvest the protein from the bacteria and purify it.
Explanation:
Well, it's obvious, because without a transport system, cells wouldn't have the nutrients and things they needed!! And waste wouldn't be able to circulate out of cells.
Answer:
the awnser is the loss of water resources in estuaries
Crossing over is important because it creates genetic variability.
<h3>What is crossing over?</h3>
It is the exchange of chromosomal segments between non-homologous chromosomes in a tetrad.
Since chromosomal segments contain genes at different loci, the exchange will increase the genetic variability of the new chromosomes.
Thus, the daughter cells carrying the recombined chromosomes will be genetically variable from not just the parent cell, but fellow daughter cells.
More on crossing over can be found here: brainly.com/question/19671756
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