Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Light is a transverse wave and this is what describes light as a wave. The correct option among all the options that are given in the question is the first option. Light is not at all similar to water and sound. It would be a big mistake to consider light as similar to the mediums water and sound. I hope the answer helps.
Iris is most likely being negatively stereotyped as being gifted.
Option D
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Explanation:</u></h3>
Many students who have proven to be gifted intellectually are likely to face struggle and negative feedback from their classmates. This is generally because they believe that the gifted student will get special treatment above the other student. Furthermore, it is evident that gifted students are likely to have different interests than their peers or much deeper knowledge of the common interest. They use terms which peers might not understand, barring them from having an effective conversation.
To maintain a balance in the class, teacher's are trained in a specific field in which they learn how to treat all the students equally, gifted or not. That helps reverse the negative stereotype or bring it down to a minimal level. As for students interacting with each other, several activities are put to place to encourage healthy interactions.
DNA<span> is a double helix, while RNA is a single helix. Both have sets of nucleotides that contain genetic information. </span>DNA<span> is a molecule that contains the instructions an organism needs to develop, live and reproduce.</span>
They also need energy to move and to stay alive
