Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
Answer: Option C) water
Explanation:
The factors affecting the distribution of organisms in aquatic biomes include:
- Light penetration: Plants life is limited to the upper layers of the ocean where light cab penetrate and be used to produce food. Thus, very few organisms are found in the ocean deep.
- Waves: the movement of surface water of the oceans, and it's direction affect the placement of organisms as they must have strong anchor or body parts suited for buoyancy
- salinity: not all organisms can survive in water with high salt content. Thus, some live in salty water, and others in fresh water or the mixture.
- Mineral content: the levels of hydrogen ion could make a water bidy alkaline or acidic thus affecting organism that can survive in it
Thus, since water ordinarilly is Universal, it is not an important factor in determining distribution of organisms in aquatic biomes
Answer:the answer is mutations
