Answer:
The rapid decolorization with ethanol or acetone
and counterstaining with safranin
When a decolorizer such as alcohol or acetone is added, it interacts with the lipids of the cell membrane. A gram-negative cell loses its outer lipopolysaccharide membrane, and the inner peptidoglycan layer is left exposed: when safranin is added they will turn red. Conversely, gram-positives retain the Cristal violet and will color in purple
Proteins are made up of a chain of 20 amino acids
<span>Observing that fibroblasts grew better in cell culture when blood serum was added to the growth medium instead of blood plasma. Thus proving that there was a substance in serum that made it easier for fibroblasts to grow.</span>
