Answer:
I'm pretty sure it is 'some species depend on disterbences'
The least associated with tRNA is 2.DNA
The use of a thermophilic DNA polymerase such as Taq polymerase prevents the denaturation of the enzyme during the heating that is important to separate the newly synthesized strand. So using this enzyme simplifies the PCR technique and increases its efficiency.
Taq DNA polymerase is highly efficient, when it reaches the optimum temperature, it becomes fully functional. It also has a half-life of over two hours (at 92°C), high amplification capacity, and it can add upto 150 nucleotides per second. It is "special" enzyme because it comes from the bacterium Thermus aquaticus, which lives in hot springs. It is therefore thermostable even at high temperatures, while other polymerases (e.g. E. coli) are not.
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Here’s your answer, Enzymes !!