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Cut open the plasmid and "paste" in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA).
Insert the plasmid into bacteria. Use antibiotic selection to identify the bacteria that took up the plasmid.
Grow up lots of plasmid-carrying bacteria and use them as "factories" to make the protein. Harvest the protein from the bacteria and purify it.
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Your answer should be A on the first two, and b on the third!!
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Answer: Your brain constantly gets signals from your body which detect the amount of oxygen and carbon dioxide in your blood. Your brain will send signals to the muscles involved in breathing and adjust your breathing rate depending on how active you are.
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