Answer:
DNA restriction enzymes cut the DNA molecule, while DNA ligases join the resulting DNA fragments
Explanation:
Transformation is a naturally occurring process by which bacteria incorporate exogenous genetic material from their surrounding environment. This process (transformation) is used for DNA cloning via plasmid vectors. In DNA cloning, transformation occurs after restriction enzymes cut the DNA at specific sequences named palindromic sequences (i.e, sequences that can be read the same in opposite direction). Restriction enzymes can generate sticky-ends, where enzymes make staggered cuts in the two strands (e.g., <em>BamH</em>), or blunt ends, where the resulting strands are of the same length (e.g., <em>HaeIII</em>). In general, sticky-end enzymes are more useful because they generate a 3' overhang in one molecule and a complementary 5' overhang in the other, increasing the yield and specificity of ligation. During ligation, a DNA ligase is used to join both DNA strands by forming phosphodiester bonds in the plasmid. Following transformation, bacteria can be selected on antibiotic plates.
Based on the facts presented about the moon, the statement that supports the idea that what we always see is the same side of the moon is statement 1. The moon rotates on its axis for about four weeks or one month.and this happens about the same period that it orbits the Earth.
Answer:
During DNA replication each parental DNA strand serves as a template to a new complementary strand. DNA polymerase is the main enzyme responsible for this process, it catalyzes the addition of nucleotides to form the new DNA chain.
The complementary nature of the DNA strands, presents a difficulty for DNA replication: DNA polymerase catalyzes the polymerization of DNA only in the 5’ to3’ -in the leading DNA strand. Thus, the opposite DNA strand, the lagging strand, faced an obstacle that is solved by the Okazaki small fragments. The primase enzyme synthesizes small RNA fragments complementary to the lagging DNA strand. These RNA fragments serve as primers for the DNA polymerase. To remove this RNA primers and form a continuous complementary lagging strand, RNase H and DNAse ligase will further cut and join the DNA again.
DNA polymerase has a higher processivity than primase, if processivity is the average number of nucleotides that it is capable to continuously add to the template strand. Primase dissociates from the template often during DNA replication as it has to constantly add new RNA primers to the strand.
The sun gets closer to the earth
