<span>When the oocyte is released from the ovary, it is transported to the uterus through the fallopian tube</span>
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
It would disrupt the CO2 cycle, since plants would no longer absorb carbon dioxixde, nor let out oxygen meaning it would also provide animals with little oxygen, and they can’t produce CO2 then either
Answer:
B) expression of inappropriate gene products
Explanation:
Most of the cancers are caused when a normal gene that code for one or other proteins involved in the regulation of the cell cycle is mutated. The mutated gene may cause the production of the faulty gene products. For example, the overproduction of products of the protooncogene that push cells through the cell cycle leads to tumor formation. Likewise, when the tumor suppressor genes are not able to produce enough product to prevent progression through the cell cycle, cancer may develop.