Question

Scientists have engineered bacteria to produce human proteins such as factor viii, a blood clotting factor to treat hemophilia. suppose the unmodified eukaryotic gene coding for factor viii is inserted directly into a bacterial chromosome. select the reasons why no expression would be seen in the bacterial cell for the factor viii gene.

Answer 1

Bacteria cannot remove intronic sequences from the gene, so if the factor VIII gene were transcribed, it would translate to a non-functional protein.

The gene will induce attenuation in the bacteria cell, which will cause a delay in the transcription and translation of the factor VIII gene