Answer:
Many plant crops need more phosphorus than is dissolved in the soil to grow optimally. In addition, crops are usually harvested and removed – leaving no decaying vegetation to replace phosphorus. Therefore, farmers replenish the phosphorus ‘pool’ by adding fertilisers or effluent to replace the phosphorus taken up by plants.
Explanation:
It serves as energy for the body the cells it helps carry out nerve ceel conduction muscle cell contraction, active transport and the production of chemical substances.
Answer:
Microfilaments, Intermediate filaments and microtubules
Explanation:
Three distinct elements make up the cytoskeleton in eukaryotic cells are:
1. Microfilaments or actin filaments which are composed of actin proteins. The functions of those filaments are: muscle contraction (myosin heads move “walk” on actin filaments), the movement of the cell, intracellular transport, maintaince of the cell shape..
2. Intermediate filaments which can be made of vimentins, keratin, lamin, desmin… Their functions are: the maintenance of cell shape, anchoring organelles, structural components of the nuclear lamina, cell-cell and cell-matrix junctions…
3. Microtubules are filaments polymers of alpha and beta tubulin. Their roles are in intracellular transport (associated with motor protein dyneins and kinesins), formation of the axoneme of cilia and flagella, formation of the mitotic spindle.
Answer:
Use a step wise process
Explanation:
In order to find the presence of the recombinant protein:
- Culture the cells that you believe harbors the plasmid for the recombination protein (+) and also cells that do not (-). This is your control.
- Extract the plasmid from both strains.
- Observe the plasmid map and the area of your insert. Use this to select the restriction enzymes at the beginning and the end of the YFG gene. You can also use a restriction site inside the gene but not in the plasmid for better control.
- Perform the restriction enzyme digest on both (+) and (-) according to your protocol and separate using gel electrophoresis.
- Observe the separate bands that you see. In the (+) there should be a band that is the size of your insert and a larger band that is the size of your plasmid control band and in the (-) there should be a single band that is the size of the plasmid but non the size of the insert.
