Soil research has shown that soil profiles are influenced by five separate, yet interacting, factors: parent material, climate, topography, organisms, and time. Soil scientists call these the factors of soil formation
A
B is wrong because mitochondria would likely absorb the stain even if it was dead.(And most cells that have been treated are dead anyway). C and D are just throwaway answers, you usually want to avoid the word "always" when drawing conclusions.
Yes, crossing over does not occur during mitosis. As mitosis is simply the duplication of cells, there is no gene variation that comes from crossing-over.