Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer: Romantic
Explanation:
In the Sternberg's theory of love, love is described on three different scales. The three different types of love described by Sternberg were based on intimacy, passion, and commitment.
Sternberg said that Romantic love involves intimacy and passion but lacks commitment. Romantic love has strong components of sexuality and infatuation. Usually romantic love is stronger at the early stages of a relationship.
Answer:
its C.specific sequences bases in DNA reproductive cells.
Explanation:
I don't know what your question is right here..? But cell division is when one cell copies DNA to make an exact copy to which the cell splits resulting in two daughter cells
Answer:
I believe it’s D
Explanation:
It’s non renewable because it doesn’t regenerate
