Cation-exchange chromatography is used when the molecule of interest is positively charged on the neutral pH. The stationary phase is than negatively charged and positively charged molecules are loaded to be attracted to it. So, positively charged peptides will elute the last.
It goes up and up and up with the idea they have unlimited resources and no threat, so it is basically like nothing ever dying and the population just going up at a unlogical rate
squeeze the fingers of the glove because of imcrease pressure