1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
PSYCHO15rus [73]
3 years ago
8

In the original Sanger sequencing method, you obtain a readout that has 4 lanes, each lane corresponding to a nucleotide. If you

get the results back and only 3 of the 4 lanes show the expected bands, which of the following would NOT be a reason for your results?a. The fluorescent color used for the label was not the correct color.b. The sample was not loaded in the well for the blank lane.c. No dNTPs were added for the blank lane's reaction.d. No sequencing primer was added for the blank lane's reaction.e. No ddNTP was added for the blank lane's reaction.
Social Studies
1 answer:
dolphi86 [110]3 years ago
7 0

Answer:

Option-1

Explanation:

DNA sequencing refers to the method which analyses the DNA and determines the sequence of nucleotide.

The Sanger method uses the same things used during the DNA replication process but an additional thing is also there called the dideoxynucleotides attached with fluorescent dye.

When thus ddNTP binds to the growing chain, then the replication process stops as ddNTP lacks the -OH group at 3'position.In the given question, since only 3 bands shows the result but one band does not it means that the florescent label has not been recognized as ddNTP label.

Thus, the problem lies in the florescent label and therefore option-1 is correct.

You might be interested in
Along her tour, Julie noticed many types of cheeses that were being aged for texture and taste. In the processing of cheese ther
In-s [12.5K]

Answer:

The correct answer is: Use of more than one food processing type (for example, canning and use of preservatives).

Explanation:

For food type like peas, there is not only one processing type that is suitable for preserving peas. Hence the correct answer is the use of more than one food processing type which include canning of peas and also using preservatives to store it. Hence, to prevent peas from spoiling, it is important to use cans, preservative use, polythene packaging and others.

4 0
3 years ago
You are researching people who have been important contributors to intelligence theory over the years, and you come across the f
pav-90 [236]

Answer: We are reading about David Weschler, who was a Romanian psychologist

Explaination: David Weschler is credited with the development of the Weschler Adult Intelligence Scale (WAIS) and the Wechsler Intelligence Scale for Children.

He was an American psychologist. He studied in City College, New York and completed his Ph.D. in 1925 from Columbia University. During World War One, he worked with US Army and developed various tests for recruits. He is known worldwide for the intelligence scales that he developed.

Further explanation

David Weschler advocated the use of non-intellective factors in IQ tests and proposed that other factors are also important for intelligent behavior, he rejected the Binet Scale. Even he didn't measure the non-intellective factors while testing but it was taken into account while test.

He developed WAI (Weschler Adult Intelligence Scale) in 1939, and this led to the development of WISC(Weschler Intelligence Scale for Children) and later WPPSI (Weschler Preschool Primary Scale of Intelligence) . All these tests follow the philosophy that " Intelligence is the global capacity to act purposefully, to think rationally and to deal effectively with the environment"

The new aspects of Weschler Scales was that it brought new methods to intelligence testing. In his methods, he divided intelligence into Verbal and Non Verbal scales. the old concept of IQ was done away with.

Learn more  

Wechsler–Bellevue Intelligence Scale: brainly.com/question/11545625

Keywords: Intelligence theory, Rational thinking, Deal effectively

8 0
3 years ago
Individuals, ____ societies, and the world change through times of conflict and cooperation
ollegr [7]

The correct answers are:

1) Cultures

2) Relgion, Culture, Economics and Politics

3) Indulgence

Explanations:

1) Culture, which comprises set of beliefs, values, norms, symbols etc., through times of conflict and cooperation. Due to globalization and cooperation, many cultures have adopted things of other cultures. One simple example could be symbols in culture. LOL, ROFL, YOLO etc. They were not cropped up as the universal phenonema. Such symbols have been adopted by other cultures. For the conflict part, you can think of two countries have conflict with each other and their beliefs about one another. How things have changed for France and Germany in terms of their beliefs about each other. I can go on, but I hope you get the gist.

2) Four distinct characteristics of any society are:

1. Religion

2. Culture

3. Economics

4. Politics

Religion is the fundamental characteristic for few societies. For example, those living in Israel or Pakistan. Without culture, a society cannot survive; so it is the integral characteristic of any society. Economics and politics also is the fundamental characteristic of any society (Examples could be the societies that support capitalism or communism or democracy or dictatorship).

3) The lessening of punishment for a wrongdoing is called an indulgence. Historically, in the Roman Catholic Church, the pope could cancel or lessen the punishments for the wrongdoings or sins, and the phenomenon is called indulgence.

7 0
3 years ago
Read 2 more answers
!!PLEASE HELP IMMEDIATELY!! Which type of climate is most prevalent in Southwest Asia?
seropon [69]

Answer:

arid

Explanation:

8 0
3 years ago
How is discipline related to guidance counseling​
valkas [14]
<h3>Answer: Discipline  is rule centred and consistence centred. Direction is commitment centred and learning centred. When chatting with kids about guidelines, I like to advance that rather than rules we could have arrangements.</h3><h3 />

Explanation:

5 0
3 years ago
Other questions:
  • Populations and the environment
    10·1 answer
  • Kai cuts her foot while hiking. She forgot to pack bandages, but she has a tube of superglue and uses that to seal the wound. Ka
    6·1 answer
  • A state of normlessness, or loss of direction, where no social ties or bonds are present, and society's control of the individua
    13·1 answer
  • place each example and correct category which activities demonstrate reserved powers and which demonstrate concurrent powers
    15·1 answer
  • Jessa just learned that she and her team are being transferred to another division. She is unhappy as she really likes her job,
    5·1 answer
  • who expressed the church's longstanding opinion that marriage is a sacrament that give grace to the couple on their journey towa
    10·1 answer
  • What continent is the sahara desert in?
    8·1 answer
  • Write an essay about the declaration of independence
    13·1 answer
  • (Amoeba sisters video recap: pedigrees and need help
    11·1 answer
  • Why did the North win the American Civil War
    15·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!