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PSYCHO15rus [73]
3 years ago
8

In the original Sanger sequencing method, you obtain a readout that has 4 lanes, each lane corresponding to a nucleotide. If you

get the results back and only 3 of the 4 lanes show the expected bands, which of the following would NOT be a reason for your results?a. The fluorescent color used for the label was not the correct color.b. The sample was not loaded in the well for the blank lane.c. No dNTPs were added for the blank lane's reaction.d. No sequencing primer was added for the blank lane's reaction.e. No ddNTP was added for the blank lane's reaction.
Social Studies
1 answer:
dolphi86 [110]3 years ago
7 0

Answer:

Option-1

Explanation:

DNA sequencing refers to the method which analyses the DNA and determines the sequence of nucleotide.

The Sanger method uses the same things used during the DNA replication process but an additional thing is also there called the dideoxynucleotides attached with fluorescent dye.

When thus ddNTP binds to the growing chain, then the replication process stops as ddNTP lacks the -OH group at 3'position.In the given question, since only 3 bands shows the result but one band does not it means that the florescent label has not been recognized as ddNTP label.

Thus, the problem lies in the florescent label and therefore option-1 is correct.

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When from a failure Heather concludes that it was her fault, that she lacks talent and will always do poorly in sculpture, she is displaying a stable, global, internal attribution about herself.

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The global and stable attribution that Heather made to herself corresponds to the fact that she believes that a result will have a permanent character, that is, will be applied broadly in a context, as in the example that she says will always do poorly in sculpture.

Therefore, the internal, stable and global attribution are psychological phenomena that can influence a person's perception of their characteristics, abilities and personality, and should be observed if it has a negative impact on an individual's life.

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brainly.com/question/14277929

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in addition to being religious centers, monasteries also provided A. military training B. educational instruction C. money-lendi
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In addition to being religious centres, monasteries also provided educational instruction.

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