Enzyme linked to the secondary antibody
an undetermined measure of antigen is joined to a surface, and after that a particular counter acting agent is connected over the surface with the goal that it can tie to the antigen. This counter acting agent is connected to a catalyst, and in the last stage a substance is included that the compound can change over to some distinguishable flag, most normally a coloration change in a concoction substrate is seen.
Answer:
25000 fragments
Explanation:
A restriction enzyme can cut a random DNA sequence once per every 4^n where n = number of bases in the recognition site of enzyme.
There are 6 bases in Xbal's recognition site (TCTAGA) so it will cut once per every 4^6 = 4096 bases (4kb).
Total genomic size = 100000kb
So expected number of fragments = Total genomic size/Size of one fragment
= 100000/4
= 25000 fragments
Answer: absolute certainty is often difficult to achieve. Scientific uncertainty generally means that there is a range of possible values within which the true value of the measurement lies.
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