Which technique can be used to determine if a sample of cells expresses both isoforms? Synthesize cDNA from Protein X mRNA using
primers overlapping exons 1 and 4, followed by gel electrophoresis and band visualization. Separate Protein X mRNA by gel electrophoresis and visualize band pattern using a DNA probe complementary to exon 3. Perform a PCR of Protein X genomic DNA using primers overlapping exons 1 and 3, followed by gel electrophoresis and band visualization. Perform a restriction digest of Protein X genomic DNA using an endonuclease that cuts in the middle of exon 2, followed by gel electrophoresis and band visualization.
Synthesize cDNA from Protein X mRNA using primers overlapping exons 1 and 4, followed by gel electrophoresis and band visualization.
Explanation:
The term isoforms refers to the ability of a cell to form different forms of a protein that will be produced through different genes, which would not be used for the same protein.
To determine whether a sample of cells expresses the two isoforms, it is necessary for the researcher to synthesize the Protein X mRNA cDNA using the primers superimposed on exons 1 and 4, followed by gel electrophoresis and band visualization.
Exons are regions of DNA that characterize some parts of the mRNA considered to be coding. Exons are used in the process mentioned above because through them it is possible to visualize the entire coding sequence of a protein. This way it is possible to know if the sample has protein isoforms.
entative) AAMC Sample FL Score Conversion ... The only way you can get to those numbers is either having a protein coded by ... a gel, and two products of different sizes tell you that both isoforms are expressed. ... of the mRNA, you would use primers for exons 1 and 4 to make DNA copies using PCR.
