Processivity of DNA polymerase may be defined as the number of nucleotides added in the single binding event. The synthesis phase of cell cycle is fully dependent on the processivity of DNA polymerase.
The processivity of DNA polymerase depends on the sliding clamp, domains of the DNA and the interaction of DNA with enzyme. Loss of hydrogen ion at 3'OH primer does not affect processivity but important for the nucleophile attack and also effect the catalytic property of enzyme.
