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juin [17]
3 years ago
12

Researchers investigated the effect of urea on the three-dimensional structure of a certain enzyme. The researchers dissolved th

e enzyme in an aqueous buffer solution and added urea to the solution. The enzyme did not appear to have a secondary or tertiary structure. The researchers carefully removed the urea from the solution and determined that the enzyme had the original secondary and tertiary structure again.Based on the results of the experiment, which of the following statements best predicts the effect of urea on the enzyme’s function?1. Function will be disrupted by adding the urea and regained by removing the urea.2. Function will be disrupted by adding the urea, but it will not be regained by removing the urea.3. Function will be gained by adding the urea and disrupted by removing the urea.4. Function will be unaffected by the addition and removal of the urea.
Biology
1 answer:
makkiz [27]3 years ago
8 0

Answer:

Function will be disrupted by adding the urea and regained by removing the urea.

Explanation:

The protein is one of the important biomolecule made of the polymers of the alpha amino acids that are linked together by peptide bonds. The protein works at their specific pH.

Denaturants disrupt the 3 dimensional structure of protein that affect their normal functioning. In the given experiment, the urea acts as denaturant and disrupt the protein structure. The removal of urea will reverse the denaturant condition and protein gain its function.

Thus, the correct answer is option (a).

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“blank” investigations are best for demonstrating cause-and-effect relationships. a. descriptive b.comparative c. experimental
FromTheMoon [43]

C. Experimental

Explanation - The cause and effect of a process would be studied in experimental investigations. However, in descriptive studies, relationship between the factors are not studies rather the detail of the process is studied. In comparative investigation, several factors are compared and its effects are analyzed.

In experimental investigations, experiments are carried out based on the hypothesis and then it is validated accordingly. Validation is a must to prove and disprove the hypothesis. Therefore, experimental data and hypothesis are related.

8 0
3 years ago
The CRISPR/Cas9 system can cleave genomic DNA at sequences other than the desired target, a phenomenon referred to as off target
Deffense [45]

Answer:

The minimum length of a sgRNA sequence to avoid off target cleavage by the CRISPR/Cas system in the fly fruit genome is 14 bases

Explanation:

We are trying to use the CRISPR/Cas system to cleavage the genome of the fruit fly (which is 1.4x10^8 bp long). Also we desire the cleavage to be unique. That means we need a target sequence long enough to be able to assume it will only appear once in the genome.

First, we should think that in every position, we can find one out of four different nucleotide (A, C, T, G). So, the probability of getting a sequence of a given length "n" will be (1/4)^n (We are assuming that the probability of finding a nucleotide in the position "i", it's independent of the nucleotide we find in any other position "j").

Also, to know how many times a sequence will appear in a genome (the expected value of occurrence), we must multiply the probability of that sequence to randomly occur by the length of the genome. For our specific example, the number of occurence of a sequence of length "n" is:

nºoccurence=[(1/4)^n]*1.4*10^8

But in this case, what we want is the expected number of times the sequence will appear to be 1, and we want to obtain the length of the target sequence (n).

Given the information above, we know that:

[(1/4)^n]*1.4*10^8 =1

[(1/4)^n]=(1/1.4*10^8)=1.4*10^-8

Then, if we want to calculate n, we can use logarithms and its properties to get:

log[(1/4)^n]=log[1.4*10^-8]

n*log[(1/4)]=log[1.4*10^-8]

n=log[1.4*10^-8]/log[(1/4)] => n=13.29 approximately.

As the sequence needs to have a natural number of elements, <u>we can conclude that using a target sequence of a minimum of 14 bases with the CRISPR/Cas system in the fly fruit genome should be enough to avoid off target cleavage.</u>

3 0
3 years ago
An organic molecule contains carbon atoms. true or false
Roman55 [17]

Answer: i’m pretty sure that it does contain carbon atoms

Explanation: most organic compounds contain a significant amount of carbon

7 0
3 years ago
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HELP ANSWER QUICK!!!!
aleksandr82 [10.1K]

Answer:

A this is the definition of homologous

D would analogous

Explanation:

8 0
3 years ago
Protists are classified into what three groups
Aleksandr-060686 [28]

<span>Protists are often classified by the characteristics that resemble those of fungi, plants, and animals</span>
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