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Drupady [299]
3 years ago
14

Investigators are studying a protein that must be phosphorylated to be activated. Which method could be used to separate the pho

sphorylated form of the protein from the form that lacks an activating phosphate group?
Chemistry
1 answer:
Roman55 [17]3 years ago
6 0

Answer:

There is two common methods to separate a phosphorylated protein from the proteins who does not have the phosphate group.

1. Western Blot test

The Western blot test is a method where we separate of the biological sample with SDS- PAGE (electrophoresis method) and then we transfer to a nitrocellulose membrane. We use a phospho-specific antibody to identify the protein of interest.

2. The ELISA test

this test is also a powerful method for measuring protein phosphorylation. Elisa test is more are quantitative than Western blot test and also shows great advantage in studies related with kinase activity. In this test we use a capture antibody specific for the desired protein, independent of the phosphorylation state. The target protein is binded to the antibody-coated plate.  and finally a detection antibody specific for the phosphorylation site analyzed is added. In these test it is possible to use colorimetric or fluorometric detection.

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How does energy from the Sun drive the cycling of matter?
Schach [20]

Answer:

solar energy drives the cycle by evaporating water from the oceans, lakes, rivers, and soil. As liquid water evaporates or transpires, it forms water vapor and clouds, where water droplets eventually gain enough mass to fall back to Earth as precipitation.

4 0
3 years ago
Can anybody help??
kvv77 [185]

The amount of heat energy added to silver to heat it from 25°C to 100°C is :

8737.5 J

<u>Given data: </u>

mass of silver ( m ) = 500 g

T1 = 25°C

T2 = 100°C

s ( specific heat of silver ) = 0.233 J/g.c

<h3 /><h3>Determine the amount of heat required </h3>

Applying the formula below for heat ( Q )

Q = ms * ΔT

   = 500 * 0.233 * ( 100 - 25 )

   = 8737.5 J

Hence we can conclude that the The amount of heat energy added to silver to heat it from 25°C to 100°C is :  8737.5 J.

Learn more about heat energy : brainly.com/question/13439286

5 0
3 years ago
When 2.85 moles of chlorine reacts with excess tin, how many moles of tin(IV) chloride are formed
svp [43]
                                Sn + 2Cl2 ------> SnCl4
from reaction                2 mol            1 mol
from the problem          2.85 mol       x mol

x=2.85*1/2≈1.43 mol SnCl4
7 0
3 years ago
Question 2 (2 points)
poizon [28]

Answer:

m_w=439.2g

Explanation:

Hello!

In this case, since the by-mass percent of a solution is a measure of the mass of the solute over the mass of the solution:

\%m/m=\frac{m_{solute}}{m_{solution}} *100\%

As we know the mass of the solution and the by-mass percent, we can compute the mass of glucose in the 480 g of solution:

m_{solute}=\frac{\%m/m*m_{solution}}{100\%}

Thus, by plugging in the data, we obtain:

m_{solute}=\frac{8.5\%*480g}{100\%}=40.8g

Finally, since the solution is made up of glucose and water, we compute the mass of water as follows:

m_w=m_{sol}-m_{solute}=480g-40.8g\\\\m_w=439.2g

Best regards!

7 0
3 years ago
We can tell when a chemical reaction occurs by looking
gregori [183]
10. ¿Con qué soñaba la autora cuando era niña? ¿Qué espera hoy?
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8 0
3 years ago
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