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Drupady [299]
3 years ago
14

Investigators are studying a protein that must be phosphorylated to be activated. Which method could be used to separate the pho

sphorylated form of the protein from the form that lacks an activating phosphate group?
Chemistry
1 answer:
Roman55 [17]3 years ago
6 0

Answer:

There is two common methods to separate a phosphorylated protein from the proteins who does not have the phosphate group.

1. Western Blot test

The Western blot test is a method where we separate of the biological sample with SDS- PAGE (electrophoresis method) and then we transfer to a nitrocellulose membrane. We use a phospho-specific antibody to identify the protein of interest.

2. The ELISA test

this test is also a powerful method for measuring protein phosphorylation. Elisa test is more are quantitative than Western blot test and also shows great advantage in studies related with kinase activity. In this test we use a capture antibody specific for the desired protein, independent of the phosphorylation state. The target protein is binded to the antibody-coated plate.  and finally a detection antibody specific for the phosphorylation site analyzed is added. In these test it is possible to use colorimetric or fluorometric detection.

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The pka of hypochlorous acid is 7.530. a 54.0 ml solution of 0.114 m sodium hypochlorite (naocl) is titrated with 0.337 m hcl. c
valkas [14]
After the addition of 6.47 mL of 0.303 M HCl. Solution :- lets first calculate the moles of the NaOCl and HCl Moles = molarity *volume in liter Moles of NaOCl = 0.100 mol per L * 0.053 L =0.0053 mol Moles of HCl =0.303 mol per L *0.00647L=0.00196 
6 0
3 years ago
1. How many ATOMS of boron are present in 2.20 moles of boron trifluoride ? atoms of boron.
maks197457 [2]

Answer:

1. How many ATOMS of boron are present in 2.20 moles of boron trifluoride? atoms of boron.

2. How many MOLES of fluorine are present in  of boron trifluoride? moles of fluorine.​

Explanation:

The molecular formula of boron trifluoride is BF_3.

So, one mole of boron trifluoride has one mole of boron atoms.

1. The number of boron atoms in 2.20 moles of boron trifluoride is 2.20 moles.

The number of atoms in 2.20 moles of boron is:

One mole of boron has ---- 6.023x10^2^3 atoms.

Then, 2.20 moles of boron has

-=2.20 mol. x 6.023 x 10^2^3 atoms /1 mol\\=13.25x10^2^3 atoms

2. Calculate the number of moles of BF3 in 5.35*1022 molecules.

(5.35x10^2^2 molecules/6.023x10^2^3)x 1mol\\=0.0888mol

One mole of boron trifluoride has three moles of fluorine atoms.

Hence, 0.0888moles of BF3 has 3x0.0888mol of fluorine atoms.

=0.266mol of fluorine atoms.

5 0
3 years ago
What is the Mr of a substance where 1 mole has a mass of 11 g?
iris [78.8K]

Answer:

A mole is the amount of pure substance containing the same number of chemical units as there are atoms in exactly

12 grams of carbon-12 (i.e., 6.023 X 1023).

Explanation:

6 0
3 years ago
A 50°C solution has a pH of 3.30. At this temperature, Kw = 5.48 x 10 9. What is the pOH of the solution?
sammy [17]

Answer:

9.93

Explanation:

Your value for Kw is incorrect. The correct value is 5.48 × 10^-14.

  pH + pOH = pKw

3.30 + pOH = -log(5.84 × 10^-14) = 13.23

          pOH = 13.23 - 3.30 = 9.93

The pOH of the solution is 9.93.

4 0
3 years ago
Read 2 more answers
Match each of the substances with the expected pH of the resulting solution when they are added to neutral water. Determine if a
Tamiku [17]
To determine the expected pH of the resulting solution of the following substances, create a balanced chemical equation of their ionization in water:

HI

HI + H2O ---> H+ + I-

It completely dissociates into H+ and I-. Due to the presence of the Hydronium Ion, the solution is acidic. 

KBr

KBr + H2O ----> HBr + KOH

The salt KBr is formed by a strong base and a weak acid, therefore, the solution it forms with water is basic. 

LiOH

LiOH + H2O ----> Li+ + OH-

It dissociates completely in water, turns into Li+ and OH-. Due to the presence of Hydroxide Ion, the solution becomes basic. 
3 0
3 years ago
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