A eukaryote is any organism whose cells contain a nucleus and other organelles enclosed within membranes. Eukaryotes belong to the taxon Eukarya or Eukaryota. The defining feature that sets eukaryotic cells apart from prokaryotic cells (Bacteria and Archaea) is that they have membrane-bound organelles, especially the nucleus, which contains the genetic material and is enclosed by the nuclear envelope.
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Cold ocean water flows from the north pole towards south and affects the climate locations near the Atlantic Ocean.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
<span>The population dynamics of the Warbler species differ from what's documented by Scott Sillett and colleagues one migration issues.
The Warbler species are non migratory species, while Scott Sillet and colleagues have been studying migratory species. The Warbler species were even taken to other islands, in some cases, in order to give them the </span>security of additional breeding populations, this because their population dynamics is not a migratory one. The studied species by Scott Sillet and colleagues, on the other hand, have migratory population dynamics: they pass their Summer time in <span>New Hampshire and and their Winter time in Jamaica.</span>
Answer:
you won't find the nucleus in prokaryotic cells
